Effects of Combined Iodinated Casein and Selenomethionine Supplementation on Thyroid Function and Immune Regulation in Hypothyroid Rats

Trends in Immunotherapy

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Effects of Combined Iodinated Casein and Selenomethionine Supplementation on Thyroid Function and Immune Regulation in Hypothyroid Rats

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https://doi.org/10.54963/ti.v10i1.1464
Orozbaeva Zhyldyzkan, Zhumabaeva Taasilkan, Abdullabekova Raissa, Meerim Taalaibekova, Salieva Kalipa, & Tugolbai Tagaev. (2026). Effects of Combined Iodinated Casein and Selenomethionine Supplementation on Thyroid Function and Immune Regulation in Hypothyroid Rats. Trends in Immunotherapy, 10(1), 71–85. https://doi.org/10.54963/ti.v10i1.1464
Volume 10, Issue 1 (March 2026): In Progress
Copyright (c) 2026 Orozbaeva Zhyldyzkan, Zhumabaeva Taasilkan, Abdullabekova Raissa, Meerim Taalaibekova, Salieva Kalipa, Tugolbai Tagaev
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Authors

  • Orozbaeva Zhyldyzkan

    Department of Pharmacy, Central Asian International Medical University, Research Institute for Medical and Biological Problems of the Southern Branch of the National Academy of Sciences of the Kyrgyz Republic, Jalal-Abad 715600, Kyrgyzstan
  • Zhumabaeva Taasilkan

    Department of General, Clinical Biochemistry and Pathophysiology, Osh State University, Research Institute for Medical and Biological Problems of the Southern Branch of the National Academy of Sciences of the Kyrgyz Republic, Osh 723500, Kyrgyzstan
  • Abdullabekova Raissa

    Department of Pharmaceutical Disciplines and Chemistry, NJSC Medical University of Karaganda, Karaganda 100008, Kazakhstan
  • Meerim Taalaibekova

    Department of Biochemistry with course of General and Bioorganic Chemistry, I.K. Akhunbaev Kyrgyz State Medical Academy named after Djumaliev A.D., Bishkek 720020, Kyrgyzstan
  • Salieva Kalipa

    Department of Chemical Engineering, Faculty of Engineering, Kyrgyz-Turkish Manas University, Bishkek 720042, Kyrgyzstan
  • Tugolbai Tagaev

    Department of Hospital Internal Medicine with a course of Hematology, I.K. Akhunbaev Kyrgyz State Medical Academy, Bishkek 720020, Kyrgyzstan

Received: 29 July 2025; Revised: 9 September 2025; Accepted: 16 September 2025; Published: 14 January 2026

Hypothyroidism, characterized by insufficient thyroid hormone production, is frequently associated with iodine deficiency. Selenium is essential for thyroid hormone metabolism and immune regulation. This study examined the effects of iodinated casein and selenomethionine supplementation on thyroid function and immune-inflammatory markers in a rat model of hypothyroidism. Thirty-six male Wistar rats were divided into four groups: control, hypothyroid (induced by thyrozol), hypothyroid rats treated with iodinated casein and selenomethionine, and hypothyroid rats treated with iodinated casein only. After 10 days of supplementation, serum levels of thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), calcitonin, and cytokines (IL-6, TNF-α, IL-10, IFN-γ) were assessed. Compared to the controls, the hypothyroid group showed elevated TSH levels, decreased T3 and T4 levels, and a pro-inflammatory cytokine profile. Treatment with both iodinated casein and selenomethionine improved TSH levels, partially restored T3 and T4 levels, and normalized cytokine levels, whereas iodinated casein alone was less effective. Calcitonin levels remained unchanged after combined supplementation. These findings suggest that supplementation with both micronutrients is more effective than iodine alone in restoring thyroid hormone balance and modulating immune responses in individuals with hypothyroidism.

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