Trends in Immunotherapy

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A Simple HPLC Method for Chlorogenic Acid Quantification in Jiruge-6 Suppositories

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https://doi.org/10.54963/ti.v9i2.908
Yilina, Yiran Bao, Nomin‑Erdene Ulambayar, & Enkhjargal Dorjbal. (2025). A Simple HPLC Method for Chlorogenic Acid Quantification in Jiruge-6 Suppositories. Trends in Immunotherapy, 9(2), 143–156. https://doi.org/10.54963/ti.v9i2.908
Volume 9 Issue 2 ( 2025)
Copyright (c) 2025 Yilina, Yiran Bao, Nomin‑Erdene Ulambayar, Enkhjargal Dorjbal
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Authors

  • Yilina

    Department of Pharmaceutical chemistry and Pharmacognosy, School of Pharmacy, Mongolian National University of Medical Sciences, Ulaanbaatar 16081, Mongolia
  • Yiran Bao

    Shenji Biotechnology Co., Ltd., Hohhot 010010, China
  • Nomin‑Erdene Ulambayar

    Department of Acupuncture and Moxibustion, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar 13270, Mongolia
  • Enkhjargal Dorjbal

    Department of Pharmaceutical chemistry and Pharmacognosy, School of Pharmacy, Mongolian National University of Medical Sciences, Ulaanbaatar 16081, Mongolia

Received: 22 April 2025; Revised: 19 May 2025; Accepted: 22 May 2025; Published: 9 June 2025

Chlorogenic acid (CGA) is a key bioactive polyphenol in Traditional Chinese Medicine (TCM) formulations including Jiruge-6 Suppositories. This study aimed to develop and validate a simple and sensitive HPLC method for quantifying CGA in these products. The optimized method utilized a C18 column with a mobile phase of acetonitrile and 0.1% phosphoric acid (15:85, v/v) at 1.0 mL/min flow rate, with detection at a 327 nm. Ultrasonic extraction with 70% methanol enhanced sample preparation efficiency. The method demonstrated excellent linearity (r² > 0.999) across the range of 1.0–100.0 μg/mL, high precision (RSD < 2.0%), and good recovery (98.2–101.5%). Detection and quantification limits were 0.30 μg/mL and 0.95 μg/mL, respectively. Analysis of five commercial batches from the same manufacturer revealed CGA content of 0.42-0.57 mg/suppository with notable seasonal variation. This validated method provides a practical approach for routine quality control of Jiruge-6 Suppositories, supporting standardization of TCM products.

Keywords:

Chlorogenic Acid; Jiruge‑6 Suppositories; HPLC; Method Validation; Traditional Chinese Medicine

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