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Bacterial Community Diversity, Richness, and Possible Immunotherapies Association in Response to Spray-Dried Porcine Plasma-Fed Diet: An Animal Model

Pirmatova Alina ORCID
Department of Internal Medicine, Osh State University, Osh 723500, Kyrgyzstan
Imad Ghanem Qasim ORCID
Department of Medical Laboratory Analysis, Al Mansour University College, Baghdad 10067, Iraq
Sura Akram Mohammed ORCID
Department of Medical Laboratory Analysis, Al-Turath University, Baghdad 10013, Iraq
Samer Shukur Mohammed ORCID
Department of Medical Laboratory Analysis, Al-Rafidain University College Baghdad 10064, Iraq
Waleed Nassar ORCID
Department of Medical Laboratory Analysis, Madenat Alelem University College, Baghdad 10006, Iraq
Nazarzoda Khusin Nazir ORCID
Department of Environmental Health, Tajik State University, Dushanbe 734025, Tajikistan

Received: 16 February 2025; Revised: 6 May 2025; Accepted: 1 July 2025; Published: 14 November 2025

Abstract

This study aims to demonstrate the significance of employing non-invasive dietary intervention to control the diversity and abundance of bacterial communities in feces. The indexing databases Scopus, PubMed/Medline, ISI Web of Science, Embase, Cochrane Central, and CINAHL were extensively searched.  Only 13 of 541 papers found in the initial searches met the criteria. Seven studies involving 210 animals were included in this meta-analysis. The SDPP group showed statistically lower Shannon (SMD: 1.20, 95% CI: 0.22–2.19, I2: 88%, p = 0.02) and Chao1 (SMD: 2.95, 95% CI: 1.99–3.91, I2: 77%, p < 0.00001) indices than the control group.  The SDPP-diet fed group showed notably lower OTU counts compared to the control group (SMD: 2.99, 95% CI: 0.67–5.32, I2: 97%, p = 0.01). The Firmicutes/Bacteroidetes ratio is reliably increased following the SDPP-fed diet (SMD: 0.68, 95% CI: 0.13–1.23, I2: 0%, p = 0.02). While noticeable differences existed between studies and difficulties were encountered in replicating basic ecological measurements, the purpose of this analysis was to identify the consistent characteristics of the gut microbiota's response to the SDPP diet, thereby pinpointing specific areas for further mechanistic research.

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