Immune cell engineering is a cutting-edge strategy in modern immunotherapy, enabling precise, personalized treatments using modified immune cells such as CAR-T and NK cells. This paper introduces an Intelligent Optimized Machine Learning (IoML) framework with Sorting Slap Swarm Optimization (SSSO) to enhance immune cell design. The IoML model employs a ranking-based Deep Neural Network (DNN) to predict therapeutic metrics—cytotoxicity, persistence, safety, and antigen specificity—from high-dimensional biological data. Public datasets including GSE120575, ImmPort, and the Single Cell Portal were used for training and validation. The SSSO algorithm efficiently explores the immune cell design space, selecting optimal configurations based on predicted performance. Simulation results highlight the IoML model as an intelligent, low-overhead solution for personalized immunotherapy. It achieved a classification accuracy of 96.8%, precision of 96.1%, recall of 95.9%, and F1-score of 96.0%, outperforming Particle Swarm Optimization, Genetic Algorithm, and Differential Evolution methods. In ranking analysis, the model attained a score of 0.92 within 30 iterations. Experimental validation confirmed optimized CAR-T cells with cytotoxicity of 96.2%, persistence of 93.8%, and apoptosis resistance of 94.3%, while reducing off-target effects to 3.2%. These findings demonstrate that the IoML–SSSO framework surpasses conventional approaches in accuracy, convergence speed, and biological relevance, effectively identifying top-performing immune cell configurations for next-generation immunotherapy platforms.