Article

Cellulase Production by Myceliophthora thermophila in Solid State Fermentation and Its Utility in Saccharification of Rice Straw

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Singh, B., Anu, A., Singh, D., Kumar, V., Kumar, V., & Malik, V. (2022). Cellulase Production by Myceliophthora thermophila in Solid State Fermentation and Its Utility in Saccharification of Rice Straw. New Energy Exploitation and Application, 1(2), 10–17. https://doi.org/10.54963/neea.v1i2.42

Authors

  • Bijender Singh
    Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendergarh-123031, Haryana, India
  • Anu Anu Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak- 124001, Haryana, India.
  • Davender Singh Department of Physics, RPS Degree College, Balana, Mahendergarh-123031, Haryana, India
  • Vinod Kumar Department of Chemistry, Central University of Haryana, Jant-Pali, Mahendergarh-123031, Haryana, India
  • Vijay Kumar Department of Botany, Shivaji College (University of Delhi), Ring Road Raja Garden, New Delhi 110027, India
  • Vinay Malik Department of Zoology, Maharshi Dayanand University, Rohtak- 124001, Haryana, India.
Optimization of cellulase production by thermophilic mould Myceliophthora thermophila BJTLRMDU3 was studied in solid state fermentation. Myceliophthora thermophila produced maximum cellulase (45.81 U/g DMR) at substrate to moisture ratio of 1:3 with 5-d old inoculum at water activity 0.95, ammonium sulfate (0.5%) and PEG 20000 (0.5%) at 45 °C using “one variable at a time” approach. Further supplementation of Tween-20 (0.5%) and K2HPO4 (0.25%) enhanced the cellulase production (56.06 U/g DMR) by M. thermophila in SSF. Optimization of saccharification by partially purified cellulase of M. thermophila (20 U), liberated maximum reducing sugars at pH 5.0 (185.56 mg/g substrate) and 60 °C (190.83 mg/g substrate) after 24 h (203.91 mg/g substrate) from sodium carbonate pretreated rice straw as compared to untreated biomass. Liberated reducing sugars were higher in sodium carbonate pretreated rice straw than untreated rice straw.

Keywords:

Rice straw M. thermophila BJTLRMDU3 Optimization Cellulase Sodium carbonate Saccharification

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