ENT Updates

Clinical Research

The effects of Sulindac on cell viability, cell cycle and angiogenesis in pharyngeal cancer cells

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https://doi.org/10.32448/entupdates.750434
Ağdaş, F., Eryılmaz, A., Gökmen Yılmaz, E., & Ergin, K. (2020). The effects of Sulindac on cell viability, cell cycle and angiogenesis in pharyngeal cancer cells. ENT Updates, 10(3). https://doi.org/10.32448/entupdates.750434
Volume 10 Issue 3 (2020)
Copyright (c) 2020 Fatih Ağdaş, Aylin Eryılmaz, Esra Gökmen Yılmaz, Kemal Ergin
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This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors

  • Fatih Ağdaş Department of Otolaryngology, Medical Faculty of Adnan Menderes University, Aydın Türkiye
  • Aylin Eryılmaz
    Department of Otolaryngology, Medical Faculty of Adnan Menderes University, Aydın Türkiye
  • Esra Gökmen Yılmaz Department of Histology and Embryology, Medical Faculty of Adnan Menderes University, Aydın, Türkiye
  • Kemal Ergin Department of Histology and Embryology, Medical Faculty of Adnan Menderes University, Aydın, Türkiye

Objective: The present study aimed to investigate the effects of Sulindac on cell viability, cell cycle and angiogenesis in pharyngeal cancer cell lines (FaDu).

Methods: FaDu cells were incubated in a medium in a 5% CO2 incubator at 37°C, after which they were proliferated and passaged. IC50 concentration was used to determine the Sulindac dose. Cells were analyzed for cell viability, cell count and cell cycle after Sulindac administration. Immunohistochemistry (vascular endothelial growth factor receptor 2) and western blot (A disintegrin and metalloproteinase with thrombospondin-like motifs 1-ADAMTS1) analyses were used for angiogenesis assessment.

Results: Cell viability decreased in pharyngeal cancer cells after Sulindac administration. In addition, FaDU cells were arrested in the G2/M phase. Sulindac was found to slightly increase vascular endothelial growth factor receptor 2 (VEGF-R2) and decrease ADAMTS1 levels in pharyngeal cancer cells.

Conclusion: Sulindac showed positive results on cell proliferation in the treatment of pharyngeal cancer cells. However, it could cause a slight increase in angiogenesis.

Keywords:

Sulindac cell cycle cell viability angiogenesis pharyngeal cancer