ENT Updates

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Analysis of tympanosclerotic plaques via atomic force microscope and scanning electron microscope

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https://doi.org/10.2399/jmu.2012003001
Aslan, H., Sinan Başoğlu, M., Ekber İlknur, A., Özbay, C., Eren, E., Oğuz Kılıç, D., Kulduk, E., & Katılmış, H. (2012). Analysis of tympanosclerotic plaques via atomic force microscope and scanning electron microscope. ENT Updates, 2(3). https://doi.org/10.2399/jmu.2012003001
Volume 2 Issue 3 (2012)
Copyright (c) 2012 Hale Aslan, Mehmet Sinan Başoğlu, Ali Ekber İlknur, Can Özbay, Erdem Eren, Duygu Oğuz Kılıç, Erkan Kulduk, Hüseyin Katılmış
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This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors

  • Hale Aslan Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Mehmet Sinan Başoğlu
    Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Ali Ekber İlknur Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Can Özbay Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Erdem Eren Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Duygu Oğuz Kılıç Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Erkan Kulduk Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey
  • Hüseyin Katılmış Department of ENT, Atatürk Training and Research Hospital, İzmir Katip Çelebi University, İzmir, Turkey

Objective: To present new information to the literature by imaging the calcospherule forming tympanosclerotic plaque's structure with atomic force microscope and to analyze the elementary structure of it with energy dispersive X-ray detector adapted to the scanning electron microscope.

Methods: Samples taken from 30 patients who underwent surgery for tympanosclerosis were retrospectively evaluated in our tertiary referral center. The surface topography and three-dimensional images of the hardest plaque was analyzed using atomic force microscopy and examined elemental composition of 5 different calcospherule in 5 different plaques using scanning electron microscopy – energy dispersive X-ray spectroscopy.

Results: In all sides of study, the rate of fronto-ethmoidal cell was 20.9%. When cases with sinonasal signs (group II and III) and control group (group I) were compared, intersiThree-dimensional analysis of the tympanosclerotic plaque using atomic force microscopy showed calcium phosphate crystalline structures. Scanning electron microscopy with energy dispersive X-ray spectroscopy was used to quantify the calcium, phosphate, carbon, nitrogen, oxygen, sodium, and magnesium present.

Conclusion: We believe that knowledge of the surface topography and elemental composition of tympanosclerotic plaque will contribute to understanding the etiology of tympanosclerosis and its treatment.

Keywords:

Tympanosclerosis plaque analysis