Antimicrobial resistance (AMR) in Gram-negative bacteria poses a severe global health threat, with metabolic reprogramming emerging as a key driver of resistance development. This study integrated transcriptomic and metabolomic analyses to investigate metabolic alterations associated with cephalosporin resistance in Escherichia coli and Klebsiella pneumoniae. Results revealed upregulation of ce...

Microbial communities (microbiomes) are integral to all ecosystems, driving critical molecular functions that shape host health and environmental processes. Recent advances in multi-omics technologies, functional genomics, and computational modeling have transformed our ability to decode the molecular mechanisms underlying microbiome function, unravel the drivers of microbiome dynamics, and qua...

Host–microbe and microbe–microbe interactions are fundamental to life on Earth, governing processes ranging from host health and disease to ecosystem function. Over the past decade, advances in molecular biology, multi-omics, and imaging technologies have unraveled the complex molecular mechanisms underlying these interactions, revealing how microbes communicate, compete, and cooperate with eac...

Microbial genomics and functional genetics have undergone transformative advances over the past decade, driven by breakthroughs in genome sequencing technologies, multi-omics profiling, and gene-function validation tools. This review synthesizes recent progress (2022–2025) in microbial genome sequencing, functional genomics, transcriptomics, proteomics, metabolomics, and gene-function discovery...

Microorganisms, including bacteria, archaea, fungi, and viruses, have evolved intricate molecular mechanisms to regulate gene expression, transduce signals, and adapt to environmental stresses. This review synthesizes recent advances (2022–2025) in microbial molecular biology, focusing on conserved and taxon-specific strategies in gene regulation, signal transduction cascades, molecular adaptat...