The clinical application of biological products is increasingly extensive, bringing good therapeutic effects for patients with a variety of immune diseases. We searched the Science Citation Index Expanded (SCI-E) database in the Web of Science Core Collection (WOSCC) and selected the highly cited papers based on biological products. The literature was analyzed based on journals, countries/regions, institutions, authors, and keywords, using VOSviewer, SCImago Graphica, and CiteSpace software to generate knowledge maps and identify hotspots and trends. The 193 highly cited papers appeared in 124 journals from 59 different countries/regions. Nature Reviews Rheumatology published most of the articles, while Nature Reviews Drug Discovery had the highest number of citations. The United States had the highest number of publications, and the top institution and author was the University of California San Diego and Fabbrocini, Gabriella. The top 5 co-occurrence keywords included drug delivery, double blind, in vitro, monoclonal antibody, and in vivo. Biological products are important adjunctive therapies for the treatment of immune-mediated inflammatory diseases. Lowering the nanotoxicity of biological products, reducing adverse events due to immunogenicity, therapeutic drug monitoring (TDM) the efficacy of biological products, and producing new substances with intrinsic antimicrobial activity may be the focus and trends for future biological products research.