ENT Updates

Clinical Research

Glutathione peroxidase and catalase enzyme gene polymorphisms in profound congenital hearing loss*

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https://doi.org/10.2399/jmu.2017003003
Tunç, O., Baysal, E., Oğuzkan Balcı, S., Mumbuç, S., Güngör Tunç, N., Pehlivan, S., & Kanlıkama, M. (2017). Glutathione peroxidase and catalase enzyme gene polymorphisms in profound congenital hearing loss*. ENT Updates, 7(3). https://doi.org/10.2399/jmu.2017003003
Volume 7 Issue 3 (2017)
Copyright (c) 2017 Orhan Tunç, Elif Baysal, Sibel Oğuzkan Balcı, Semih Mumbuç, Nihal Güngör Tunç, Sacide Pehlivan, Muzaffer Kanlıkama
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This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors

  • Orhan Tunç
    Department of Otorhinolaryngology, Cengiz Gökçek Hospital, Gaziantep, Turkey
  • Elif Baysal Department of Otorhinolaryngology, Balat Hospital, Istanbul, Turkey
  • Sibel Oğuzkan Balcı Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
  • Semih Mumbuç Department of Otorhinolaryngology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
  • Nihal Güngör Tunç Clinic of Otorhinolaryngology, 25 Aralık Hospital, Gaziantep, Turkey
  • Sacide Pehlivan Department of Medical Biology, Faculty of Medicine, Istanbul University, Istanbul, Turkey
  • Muzaffer Kanlıkama Department of Otorhinolaryngology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey

Objective: The aim of this study was to search the codon 200 polymorphism on the glutathione peroxidase 1 gene (GPX1) and A/T changes on the promoter region of the catalase gene (CAT) in cochlear implant patients with congenital profound hearing loss.

Methods: Sixty-five cochlear implant patients with congenital hearing loss and 100 age- and gender-matched healthy volunteers were evaluated between 2011 and 2013. Genomic DNA was extracted from peripheral blood samples by using the salting out procedure. The T/A polymorphism in the promoter region of the CAT gene (rs7943316) and GPX1 gene codon 200 proline to leucine substitution (rs1050450) were determined by polymerase chain reaction and restriction fragment length polymorphisms.

Results: No statistically significant difference was found in CC and CT genotypes in codon 200 on GPX1 (CC, p=0.10; CT, p=0.48) However, there was a statistically significant difference in the TT genotype (p=0.04). In the CAT promoter region, there was no statistically significant difference between the patients and control groups (AA, p=0.41; TA, p=0.16; TT, p=0.08).

Conclusion: As a conclusion, the TT genotype on the GPX1 codon 200 may have a relationship with congenital profound sensorineural hearing loss.

Keywords:

Catalase cochlear implant congenital sensorineural hearing loss glutathione peroxidase polymorphism